Buy article

Mucin 5AC is significantly upregulated in exosomes from the nasal lavage fluid and may promote the expression of COX-2, VEGF and MMP-9: an implication in nasal polyp pathogenesis

Volume: 59 - Issue: 3

First page: 328 - Last page: 336

L.-F. Wang - C.-H. Lee - S.-S. Liang - C.-C. Hung - Y.-R. Wu - C.-Y. Chien - C.-H. Lee - J.Y.-F. Chen

BACKGROUND: Exosomes are critical mediators of intercellular communication and could be involved in many human diseases; however, little is known about the role of exosomes in nasal polyps (NP).
METHODS: Exosomes in nasal lavage fluids (NLF) were isolated by ultracentrifugation. Exosome identity was validated by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and specific exosomal markers. The exosome proteome was revealed by LC-MS/MS, and the expression of the candidate exosomal protein, mucin 5AC, was confirmed by Western blot analysis and immunohistochemistry (IHC). Cellular uptake of the exosomes was monitored by fluorescence confocal microscopy and the ensuing effects on COX-2, VEGF and MMP-2/MMP-9 were determined by Western blotting, ELISA and gelatin zymography, respectively.
RESULTS: Mass spectrometry analysis and subsequent verification by Western blotting identified that mucin 5AC was significantly upregulated in exosomes from NLFs of NP patients. Moreover, the expression of mucin 5AC was increased in the tissue specimens of the NP patients. Functional assays suggest that the mucin 5 AC-enriched exosomes could be effectively taken up by chronic rhinosinusitis without NP (CRSsNP)-derived fibroblasts, the control cells, resulting in a significant increase in the expression of COX-2, VEGF and MMP-9.
CONCLUSIONS: Mucin 5AC, the major airway mucin, cannot only be carried and transferred by nasal exosomes, but may also promote tissue remodeling and angiogenesis and thus could be a potential therapeutic target of NP.

Rhinology 59-3: 328-336, 2021

To see the issue content and the abstract you do not have to login

Please login to download the full articles

If you do not have a subscription to Rhinology please consider taking one.

Click here to become a member of the European Rhinologic Society and a subscriber to the journal `RHINOLOGY`, from 2023. Subscription including membership fee: Euro 135.-

  1. Situs Slot KayaMendadak88
  2. Situs Slot Online Terbaik Dan Terpercaya No 1
  3. Slot Gacor
  4. Slot Terbaru: https://www.shaptahik.com/slot-terbaru.php
  5. Slot Pragmatic Play
  6. Situs Slot Online Terpercaya
  7. Kumpulan Situs Judi Slot Terpercaya
  8. Game Slot Online
  9. Judi Slot Online Jackpot Terbesar
  10. Situs Judi Slot Online Terpercaya 2021
  11. Situs Slot Online Indonesia
  12. SLOT88
  13. RAJASLOTXO
  14. Slot Joker123
  15. Situs Slot Online Terbaik
  16. Daftar Situs Slot Online
  17. Agen Slot Online Resmi
  18. Slot Deposit Pulsa
  19. Situs Slot Gacor
  20. Situs Judi Slot Online Terbaik & Judi Online 2021
  21. Agen Slot Online Terpercaya
  22. Daftar Situs Judi Slot Terbaik Dan Terpercaya No 1
  23. Pragmatic Play Indonesia
  24. Daftar Situs Judi Slot Online Gampang Menang Terbaik 2021
  25. AloJudi Slot
  26. KayaMendadak88 Slot
  27. Situs Judi Slot Online: http://happy.daa.jp/slot-online/
  28. Situs Judi Slot Online Joker Slot Gaming
  29. Situs Slot Gacor 2022
  30. Slot Gacor Gampang Menang
  31. Link Alternatif: https://nimafadavibeats.com/
  32. Situs Judi Slot Online Indonesia: https://hgj.nbc.mybluehost.me/
  33. Situs Judi Slot Mudah Menang
  34. Situs Slot Online Terbaik dan Terpercaya
  35. Situs Judi Slot Online Terpercaya
  36. slot
  37. Situs Judi Slot Online Jackpot Terbesar
  38. Situs Judi Slot Terbaik Dan Terpercaya No 1
  39. Judi Slot Online Jackpot Terbesar Gampang Menang
  40. Slot Online Terlengkap
  41. Slot Pulsa
  42. Slot Deposit DANA
  43. SLOT88 Pulsa
  44. Situs Judi Online Resmi Aman Dan Terpercaya 2022
  45. https://www.nubekasi.id/
  46. Forum Slot Online Indonesia
  47. Daftar Terbaru Situs Judi Slot Online Terpercaya 2022
  48. Situs Judi Slot Online Indonesia Terpercaya
  49. Situs Judi Slot Online Terpercaya di Indonesia 2022
  50. situs judi slot gacor online terpercaya
  51. Situs Judi Slot Online Terbaik dan Terpercaya 2022
  52. Joker123 Slot
  53. Daftar Situs Judi Slot Online Deposit Pulsa Terbaik
  54. Daftar Situs Judi Slot Online Gacor Gampang Menang 2022
  55. Mesin slot gacor 88
  56. Mesin slot
  57. Slot Hacker
  58. Situs Judi Slot Online Resmi 2021
  59. Slot Via Dana
  60. Situs Judi Slot Online Terpercaya No 1
  61. Link Alternatif Joker123
  62. Situs Judi Slot Mudah Menang